*We provide cell-specific transfection reagents with specific protocols for hard-to–transfect cells.
Most commonly used cell types are efficiently trasnfected with Targefect THE VIROFECT ADVANTAGE - Virofect is a adenovirus derived enhancer formulation used to increse gene delivery into hard-to-trasnfect cell types.
Why Targefect?
Efficient gene transfer accomplished using unique approaches to gene delivery!
- Use of unique reagent that escort genes directly to the nucleus and mediate disrupt ion of endosomes.
- Use of simple easy to use lipid formulations that efficiently mediate gene delivery into many hard-to transfect cell types.
- Use of adenovirus-based enhancer reagents that exploit adenoviral receptors on the cell surface for efficient gene transfer. This method yields high efficiencies of gene transfer as well as increased expression of the transgene.
- We provide optimized cell-specific protocols for most cell types
- Simplified protocols provided for high throughput applications upon request.
Various factors contribute to the efficient performance of the Targefect product line. The Tarefect F-2 reagent, Peptide Enhancer and Virofect directly take DNA to the nucleus because of their nuclear escorting proterties. Since it is well documented that nuclear import enhances gene expression, this usually results in higher transgene expression. The confocal image below showing a picture of human microvascular endothelial cells transfected with a green fluorescent protein expression vector using Targefect F-2 in combination with the Peptide Enhancer clearly demonstrates efficient nuclear delivery. Cells have been counterstained with a red fluorescent dye to visualize cells. (Courtesy of Drs Steve Duffy and Dr. J Murphy, UT Southwestern Medical Center, Dallas, TX)
CLICK ON THE LINKS BELOW FOR MORE INFO