In Vivo-Targefect

 In Vivo-Targefect is a sterile formulation of non-ionic polymers which mediates efficient intra-muscular gene transfer without any immunogenic effects. The transfection protocol is extremely simple and involves simple mixing of the Targefect formulation with DNA and incubation for 30 min at RT. The product of the expressed transgene can be efficiently secreted into circulation by using expression vectors containing a secretory signal. Efficient intra-muscular gene delivery in mice is seen injecting as little as 20 ug DNA (complexed with 7 ul of Targefect) although improvement is observed using higher amounts of DNA (upto 50 g/mouse).

CATALOG #

SIZE

Number of injections per mouse

PRICE

       

IN-VIVO-TARG-001

200 ul

10 injections

$600

IN-VIVO-TARG-002

600 ul

30 injections

$1200

IN-VIVO-TARG-003

3.2 ml

160 injections

$3500

Ordering: To place an order please use our order form at the following link https://www.targetingsystems.net/product-price-list.php

Product Brochure: See link below https://targetingsystems.net/pdf/NEW%20IN%20VIVO-TARGEFET%20Brochure%202021.pdf

Product Protocol: See link below https://targetingsystems.net/pdf/IN%20VIVO-TARGEFET%20Protocol%202021.pdf

Storage/Stability : Stable at 4 o C for 1 year from date of purchase

Modes of Administration: Intra-muscular injection

     

    Advantages:

  • Simple transfection protocol, no electroporation or pulsed electrical field.
  • Sustained Gene transfer for at least 1 month
  • Functionality validated in mice
  • No detectable immune response
  • Successful gene delivery via multiple routes of administration
  • Efficient secretion of transgene from transduced muscle tissue using appropriate secretory signals
  • Expression vectors available for efficient secretion of transgene following IM injection

DATA: Efficient, sustained intra-muscular gene expression using In Vivo-Targefect

 

g1mice invivotransfection

Figure 1: Expression of a luciferase following intramuscular injection of an expression vector pCMV-Luc in mice: Panel A (Left) : Bioluminescence imaging of firefly luciferase expression at day 2, 12 and in transduced mice by injecting firefly luciferin at day 2 and 16 after intramuscular injection of 50 ug DNA (in 10 ul water) mixed with 22.5 ul water and then complexed with 10 ul of In Vivo-Targefect , incubated at RT for 30 mins and then injected intra-muscularly into the mouse calf muscle. Panel B (Right): Quantitation of firefly luciferase expression (photons/sec) at different times following gene transfer. Results show sustained gene transfer for 27 days following intramuscular gene delivery

 

Systemic gene expression of a secreted gene (Gaussia luciferase) delivered by intramuscular gene transfer using In Vivo-Targefect

day2mouse f2b-tif

Figure 2: Imaging of systemic Gaussia luciferase expression and intramuscular firefly luciferase expression in vivo following intramuscular injection of a dual luciferase-expressing lentiplasmid: Left Panel: Bioluminescent imaging of secreted Gaussia luciferase expression in transduced mice by injecting coelenterazine at day 2 and 16 after intramuscular injection of 20 ug DNA (in 20 ul water) complexed with 10 ul of In Vivo-Targefect , incubated at RT for 30 mins and then injected intra-muscularly into the mouse calf muscle. The expression vector used in these studies was a lentiplasmid co-expressing two different luciferase reporters. intracellular red-emitting firefly luciferase and secreted Gaussia luciferase. Right Panel: Bioluminescent imaging of the same mice with firefly luciferin to demonstrate intramuscular expression of the non-secreted red-emtiting firefly luciferase

 

f3

Figure 3: Quantitation of Gaussia luciferase expression (photons/sec) at different times following gene transfer. Results show sustained gene transfer for 30 days following intramuscular gene delivery