CATALOG # |
SIZE |
Number of injections per mouse |
PRICE |
IN-VIVO-TARG-001 |
200 ul |
10 injections |
$600 |
IN-VIVO-TARG-002 |
600 ul |
30 injections |
$1200 |
IN-VIVO-TARG-003 |
3.2 ml |
160 injections |
$3500 |
Ordering: To place an order please use our order form at the following link https://www.targetingsystems.net/product-price-list.php
Product Brochure: See link below https://targetingsystems.net/pdf/NEW%20IN%20VIVO-TARGEFET%20Brochure%202021.pdf
Product Protocol: See link below https://targetingsystems.net/pdf/IN%20VIVO-TARGEFET%20Protocol%202021.pdf
Storage/Stability : Stable at 4 o C for 1 year from date of purchase
Modes of Administration: Intra-muscular injection
DATA: Efficient, sustained intra-muscular gene expression using In Vivo-Targefect
Figure 1: Expression of a luciferase following intramuscular injection of an expression vector pCMV-Luc in mice: Panel A (Left) : Bioluminescence imaging of firefly luciferase expression at day 2, 12 and in transduced mice by injecting firefly luciferin at day 2 and 16 after intramuscular injection of 50 ug DNA (in 10 ul water) mixed with 22.5 ul water and then complexed with 10 ul of In Vivo-Targefect , incubated at RT for 30 mins and then injected intra-muscularly into the mouse calf muscle. Panel B (Right): Quantitation of firefly luciferase expression (photons/sec) at different times following gene transfer. Results show sustained gene transfer for 27 days following intramuscular gene delivery
Systemic gene expression of a secreted gene (Gaussia luciferase) delivered by intramuscular gene transfer using In Vivo-Targefect
Figure 2: Imaging of systemic Gaussia luciferase expression and intramuscular firefly luciferase expression in vivo following intramuscular injection of a dual luciferase-expressing lentiplasmid: Left Panel: Bioluminescent imaging of secreted Gaussia luciferase expression in transduced mice by injecting coelenterazine at day 2 and 16 after intramuscular injection of 20 ug DNA (in 20 ul water) complexed with 10 ul of In Vivo-Targefect , incubated at RT for 30 mins and then injected intra-muscularly into the mouse calf muscle. The expression vector used in these studies was a lentiplasmid co-expressing two different luciferase reporters. intracellular red-emitting firefly luciferase and secreted Gaussia luciferase. Right Panel: Bioluminescent imaging of the same mice with firefly luciferin to demonstrate intramuscular expression of the non-secreted red-emtiting firefly luciferase
Figure 3: Quantitation of Gaussia luciferase expression (photons/sec) at different times following gene transfer. Results show sustained gene transfer for 30 days following intramuscular gene delivery