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Products > LiveResponse™
Our latest offering - LiveResponse™In our search for multiplexed assays that increase both assay speed and sensitivity we have developed LiveResponse™ a panel of 5 secreted ultra-sensitive luciferase reporters - Gaussia luciferase (emission max 482 nm), Cypridina luciferase (emission max 463 nm), Blue-shifted Renilla luciferase emission max 467 nm, Green Renilla luciferase (emission max 527 nm) and a Red Italica firefly luciferase (emission max 613 nm). These significantly brighter luciferases (Gaussia luciferase is over a 1000-fold brighter than the Photinus firefly luciferase and the red-emitting Luciola luciferase is a 1000 times brighter than it‘s native counterpart) offer increased sensitivity in screening applications involving analysis of weak promoters or hard-to-transfect cells. The green variant of Renilla luciferase that is a component of the LiveResponse™ system has 100-fold greater signal intensity and offers improved stability of the luminescent signal both in vitro and in vivo. The LiveResponse™ system (a collection of 5 luciferase reporters) and assay reagents for the same) offers flexibility in assay format i.e. Different reporters can be assayed individually using separate aliquots of the supernatant or up to three luciferases can be assayed in the same sample of supernatant and the relative activities of the different luciferases can be spectrally resolved using appropriate filters. Advantages
Product InformationLiveResponse-A panel of 5 novel ultrasensitive luciferase reporters for cell-based HTS applications American Society for Cell Biology meeting , San Diego, December, 2009 Presentations describing use of the LiveResponse reporters : Technical resourcesRelated applications |