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Features & Sequences of pCMV-GrRenLuc Vector Key Features : CMV Promoter : 18 - 862 Green Renilla Luc cds : 913 - 1905 f1 ori : 2056 - 2362 SV40ER : 2472 - 2708 SV40 pro : 2494 - 2763; 2615 - 2816 SV40 ori : 2662 - 2739 Neomycin cds : 2878 - 3672 ColE1 replicon : 4403 - 5022 Beta-Lactamase cds : 5177 - 6037 (complement) Beta-Lactamase Promoter: 6079 - 6107 pCMV Green Renilla Luciferase plasmid Sequence : ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 901 gagctcggat ccatgttgtt gaaagttgtg tttgctattg gatgtatcgt agtgcaggct 961 atggcctcaa aagtgtacga tccggagcag cggaagagga tgatcacggg gccccaatgg 1021 tgggcacgat gcaagcagat gaatgtgttg gacagtttca ttaactacta cgacagcgag 1081 aaacacgcgg agaacgcagt gatattcctg cacggcaatg caaccagtag ctatctgtgg 1141 agacacgtgg tgcctcatat tgagccggtc gctagatgca ttattcccga tcttattgga 1201 atggggaaat ccggaaagag tggaaatgga tcatataggc tcctcgatca ttataaatat 1261 ctgactgctt ggtttgaatt gctcaatctg cccaagaaaa tcatctttgt aggacatgat 1321 tggggctccg cccttgcttt tcattatgcc tatgaacacc aggatcggat caaggctatt 1381 gttcacatgg agagcgtggt ggatgtgatt gaatcatgga tgggttggcc ggatatagaa 1441 gaagagctgg cgctgattaa atctgaggag ggcgagaaga tggtactcga aaataacttc 1501 tttgtcgaga cggtactgcc cagtaagatc atgcgcaaac tggagcctga agagtttgcg 1561 gcttacctgg aacccttcaa ggagaaggga gaggtgagga gaccgaccct gtcatggcct 1621 cgggaaattc cgctggtcaa aggagggaag ccagacgtcg tcgccattgt ccggaattac 1681 aacgcttacc tccgcgctag tgacgacctg cctaaactct tcatcgaatc agatcctggt 1741 ttctttagta acgccatcgt cgagggcgcc aagaagtttc caaacaccga atttgttaaa 1801 gtcaaaggac ttcacttcct ccaggaggat gcgcccgatg aaatgggaaa gtatatcaaa 1861 tccttcgtgg agagggtctt gaagaatgag cagaggtcca tctagtctag aaataattct 1921 tactgtcatg ccaagtaaga tgcttttctg tgctgcaata gcaggcatgc tggggatgcg 1981 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac 2041 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 2101 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 2161 ttcgccggct ttccccgtca agctctaaat cggggcatcc ctttagggtt ccgatttagt 2221 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 2281 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 2341 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 2401 gggattttgg ggatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 2461 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 2521 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 2581 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 2641 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 2701 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag 2761 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg 2821 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg 2881 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc 2941 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg 3001 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag 3061 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc 3121 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat 3181 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg 3241 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc 3301 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag 3361 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc 3421 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc 3481 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata 3541 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc 3601 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac 3661 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc 3721 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt 3781 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc 3841 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 3901 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 3961 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat 4021 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 4081 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 4141 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 4201 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 4261 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 4321 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 4381 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 4441 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 4501 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 4561 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac 4621 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 4681 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 4741 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 4801 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 4861 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 4921 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 4981 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 5041 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 5101 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 5161 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 5221 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 5281 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 5341 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 5401 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 5461 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 5521 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 5581 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 5641 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 5701 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 5761 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 5821 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 5881 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 5941 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 6001 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 6061 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 6121 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc Please note :
For question pertaining to commercial use of the Gaussia luciferase gene please contact LI Bryan li@prolume.com, and read the tear away license that accompanies the Gaussia luciferase expression vectors. |