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Innovations in Drug Discovery

The largest selection of ultra-sensitive luciferase reporters in the market.
Seven colors of the spectrum. 6-1000 times brighter then commercially available Photinus and Renilla luciferases.

Color	Luciferase	Source	Substrate	Emission Max	Secreted	Intracellular	Relative Brightness (compared to Photinus Pyralis)
Violet*	Vargula (Cypridina) Luciferase Read More	Vargula Hilgendorfi	Vargulin	395 nm	Yes	Some activity	40-fold
Indigo	Vargula (Cypridina) Luciferase Read more info.	Vargula Hilgendorfi	Vargulin	460 nm	Yes	Some activity	40-fold
Cyan Blue	Gaussia Luciferase Read more info.	Gaussia Princeps	Coelenterazine	482 nm	Yes	Some activity	1000-fold
Cyan Blue	Gaussia Luciferase Read more info.	Gaussia Princeps	Coelenterazine	482 nm	No	Yes	1000-fold
Blue*	Renilla Luciferase Read more info.	Renilla reniformis	Coelenterazine	482 nm	Yes	Yes	6-fold
Green*	Renilla Luciferase Read more info.	Renilla reniformis	Coelenterazine	530 nm	Yes	Yes	20-fold
Green	Firefly Luciferase Read more info.	Luciola Italica	Firefly Luciferin	560 nm	No	Yes	10-fold
Red	Firefly Luciferase Read more info.	Luciola Italica	Fiefly Luciferin	609 nm	No	Yes	100-fold

*Products to be released soon

Product Information

Gaussia Luciferase Assay System
Luciola Italica Firefly luciferase assay system
Vargula (Cypridina) Luciferase Assay system
Renilla luciferase assay system

Luciferase Expression Vectors :
Plasmid expression vectors expressing the luciferases shown above in mammalian expression vectors under control of the CMV promoter or as promoterless (Basic) vectors which enabling subcloning your promoter of interest into a multiple cloning site upstream of the luciferase reporter.

Luciferase Assay Reagents :
(single assays, dual and triple luciferase reporter assays: Our unique formulation of single solution based multiplexed luciferase assay present a tremendous advantage for applications in drug discovery by reducing assay time and cost by assaying different luciferases based on differences in emission spectra by using appropriate filters.

Lenti-Luciferase Technology :
Lentiviruses expressing Gaussia lcuiferase or the red emitting firefly luciferase offer several advantages in cell tracking, bioluminescent imaging etc and circumvent the cumbersome task of transfectign cells.

Applications in Gene silencing :
The psiScreen system (based on secreted luciferases) enables rapid screening of siRNAs/ study regulation of microRNA in vitro and in vivo.

Citations

Articles citing use of our luciferase reporters

Drug discovery made easier through custom solutions
Also available are plasmid constructs NFKappaB-luciferase or CREB-luciferase expressing any one of our luciferase reporters.

CREB reporter Stable Cell lines (HEK-293 and ChoK1)
These cell lines are useful for high throughput analysis of cAMP and CREB acitivation, enable profiling of GPCR acitivity and screening of compounds affecting CREB-dependent pathways .

NFkB-luciferase Cell lines: These cell lines are designed for monitoring the activity of NFkB transcription factor in cell-based assays. Please enquire (1-866-620-4018) for our custom offerings…

Additional Resources

Poster presentation
Product review-Triple luciferase reporter assays : The Triple luciferase reporter assays review will be available in about a week in the online journal biocompare.com
Gene reporter assays (article)
Press release on in vivo imaging applications
Gaussia based ER Stress Monitoring Kit — now available from Prolume, AZ.
Slide presentation on overview of our drug discovery product line
LeniGlo and LentiPower

We are rapidly expanding our product offerings in this group. Please check back soon for updates.