Efficiency of protein/peptide delivery into different cell types using Profect reagents:
The following table is compiled from our own data as well as data provided by our customers.
(Over 50 cell tpes have been transfected, this is a list of selected cell types of common interest)
| Cell Lines/Model |
Cell Type |
Macromolecule Delivered |
Transfection Efficiency |
| Human mesenchymal stem cells |
Primary human mesenchymal stem cells |
Transcritption factors, histone
proteins |
70--80% |
| CD34 positive hematopoietic cells |
Hematopoietic stem cells |
Peptides |
70% |
| Raw 264.7 |
Macrophage cell line |
Peptides, proteins (virulence factors), Antibody, enzymes |
70-85% |
| MCF-7, MDA |
Mammary breast cancer cell lines |
Caspase 3, histone, Beta galactosidase |
70-80% |
| A549 |
Lumg carcinoma cell lines |
Antibodies |
70% |
| Human
Keratinocytes |
Primary human keratinocytes |
Beta galactosidase
|
70% |
| RPE |
Retinal pigmented epithelial cell lines |
Enzymes |
70-90% |
| HLECs |
Human lens epithelial cells |
Enzymes |
80% |
| HUVECs |
Human umbilical vein endothelial cells |
Antibodies, histone |
70-80% |
| CV-1 |
Monkey kidney |
Proteins/peptides |
80% |
| HeLa |
Human cervical carcinoma |
Histone, antibody, peptide, Beta galactosidase |
80% |
| OVCAR |
Ovarian cancer cell line |
Antibody |
80%-85% |
| HEK-293 |
Human Embryonic Kidney |
Peptides |
80% |
| NIH 3T3 |
Mouse fibroblast |
Antibody |
70% |
| COS-7 |
Monkey kidney |
Antibody, enzymes |
80-90% |